Journal: bioRxiv
Article Title: Evidence for a RIPK1-independent survival mechanism for CASPASE-8 in αβ T cells
doi: 10.1101/2025.03.27.645705
Figure Lengend Snippet: Thymi from Casp8ΔT CD2 mice (n=19) and Cre -ve litter mates (n=14) were enumerated and analysed by flow. (A) Density plots are representative expression of CD4 vs CD8 by live gated thymocytes. Bar charts show the numbers of total thymocytes, CD4 − CD8 − DN thymocytes and CD4 + CD8 + DP thymocytes. (B) Dot plots are of CD44 vs CD25 expression by CD4 − CD8 − TCRb − HSA hi thymocytes from representative mice. Bar charts summarise numbers of different DN subsets. (C-D) Density and dot plots of HSA vs CD62L are from CD4 + CD8 − TCRβ + (C) and CD4 − CD8 + TCRβ + (D) live thymocytes. Bar charts summarise the total numbers of SP, immature HSA hi CD62L lo and mature HSA lo CD62L hi SP subsets. (E) Density plots are of HSA vs CD1d-α-GalCer (CD1dTet) binding to total live thymocytes. CD4 vs CD8 expression is for CD1dTet + cells. HSA vs CD44 expression is shown for CD4 + CD8 − CD1dTet + (CD4 NKT) cells and CD4 − CD8 − CD1dTet + (DN NKT) cells. Bar charts summarise total numbers of CD4 NKT and DN NKT recovered from mice. Data are pooled from multiple batches of mice analysed.
Article Snippet: Panels used the following mAb: PE-Cy7-conjugated antibody against CD25 (eBioscience), PE-conjugated antibody against CD127 (ThermoFisher Scientific), BV785-conjugated CD44 antibody (Biolegend), BV421-conjugated antibody against CD4 (Biolegend), BUV395-conjugated antibody against CD8 (BD Biosciences), BV711-conjugated antibody against CD24 (BD Biosciences), PerCP-Cy5.5-conjugated antibody against TCR (Cambridge Biosciences), FITC-conjugated antibody against CD5 (eBiosciences), BV650-conjugated antibody against CD45.1 (Biolegend), FITC-conjugated antibody against CD45.2 (ThermoFisher Scientific), APC-conjugated antibody against CD49d (Biolegend), FITC-conjugated antibody against Ki67 (ThermoFisher Scientific).
Techniques: Expressing, Binding Assay