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bd biosciences cd16 pe cy7  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec bd biosciences cd16 pe cy7
    Bd Biosciences Cd16 Pe Cy7, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bd biosciences cd16 pe cy7/product/Miltenyi Biotec
    Average 96 stars, based on 22 article reviews
    bd biosciences cd16 pe cy7 - by Bioz Stars, 2026-02
    96/100 stars

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    Image Search Results


    Thymi from Casp8ΔT CD2 mice (n=19) and Cre -ve litter mates (n=14) were enumerated and analysed by flow. (A) Density plots are representative expression of CD4 vs CD8 by live gated thymocytes. Bar charts show the numbers of total thymocytes, CD4 − CD8 − DN thymocytes and CD4 + CD8 + DP thymocytes. (B) Dot plots are of CD44 vs CD25 expression by CD4 − CD8 − TCRb − HSA hi thymocytes from representative mice. Bar charts summarise numbers of different DN subsets. (C-D) Density and dot plots of HSA vs CD62L are from CD4 + CD8 − TCRβ + (C) and CD4 − CD8 + TCRβ + (D) live thymocytes. Bar charts summarise the total numbers of SP, immature HSA hi CD62L lo and mature HSA lo CD62L hi SP subsets. (E) Density plots are of HSA vs CD1d-α-GalCer (CD1dTet) binding to total live thymocytes. CD4 vs CD8 expression is for CD1dTet + cells. HSA vs CD44 expression is shown for CD4 + CD8 − CD1dTet + (CD4 NKT) cells and CD4 − CD8 − CD1dTet + (DN NKT) cells. Bar charts summarise total numbers of CD4 NKT and DN NKT recovered from mice. Data are pooled from multiple batches of mice analysed.

    Journal: bioRxiv

    Article Title: Evidence for a RIPK1-independent survival mechanism for CASPASE-8 in αβ T cells

    doi: 10.1101/2025.03.27.645705

    Figure Lengend Snippet: Thymi from Casp8ΔT CD2 mice (n=19) and Cre -ve litter mates (n=14) were enumerated and analysed by flow. (A) Density plots are representative expression of CD4 vs CD8 by live gated thymocytes. Bar charts show the numbers of total thymocytes, CD4 − CD8 − DN thymocytes and CD4 + CD8 + DP thymocytes. (B) Dot plots are of CD44 vs CD25 expression by CD4 − CD8 − TCRb − HSA hi thymocytes from representative mice. Bar charts summarise numbers of different DN subsets. (C-D) Density and dot plots of HSA vs CD62L are from CD4 + CD8 − TCRβ + (C) and CD4 − CD8 + TCRβ + (D) live thymocytes. Bar charts summarise the total numbers of SP, immature HSA hi CD62L lo and mature HSA lo CD62L hi SP subsets. (E) Density plots are of HSA vs CD1d-α-GalCer (CD1dTet) binding to total live thymocytes. CD4 vs CD8 expression is for CD1dTet + cells. HSA vs CD44 expression is shown for CD4 + CD8 − CD1dTet + (CD4 NKT) cells and CD4 − CD8 − CD1dTet + (DN NKT) cells. Bar charts summarise total numbers of CD4 NKT and DN NKT recovered from mice. Data are pooled from multiple batches of mice analysed.

    Article Snippet: Panels used the following mAb: PE-Cy7-conjugated antibody against CD25 (eBioscience), PE-conjugated antibody against CD127 (ThermoFisher Scientific), BV785-conjugated CD44 antibody (Biolegend), BV421-conjugated antibody against CD4 (Biolegend), BUV395-conjugated antibody against CD8 (BD Biosciences), BV711-conjugated antibody against CD24 (BD Biosciences), PerCP-Cy5.5-conjugated antibody against TCR (Cambridge Biosciences), FITC-conjugated antibody against CD5 (eBiosciences), BV650-conjugated antibody against CD45.1 (Biolegend), FITC-conjugated antibody against CD45.2 (ThermoFisher Scientific), APC-conjugated antibody against CD49d (Biolegend), FITC-conjugated antibody against Ki67 (ThermoFisher Scientific).

    Techniques: Expressing, Binding Assay

    Lymph nodes and spleen from the cohorts of mice described in were enumerated and analysed by flow. (A) Density plots are of CD4 vs CD8 by TCRβ + lymph node cells, CD62L vs CD44 by either CD4 + TCRβ + CD25 − or CD8 + TCRβ + subsets. Gates used to identify CD4 + naive, CM and EM subsets are labelled in red. Naive, CM and VM CD8 subsets were identified amongst CD8 + CD62L hi TCRβ + cells using CD49d and CD44 as indicated. (B) Bar charts summarised the total numbers of the indicated CD4 and CD8 subsets from total lymph nodes and spleen combined. (C-D) Density plots are of SSC vs Ki67 by the indicated subsets of CD4 and CD8 T cells in thymus and lymph nodes. Bar charts summarise the frequency of Ki67 + cells amongst the indicated subsets.

    Journal: bioRxiv

    Article Title: Evidence for a RIPK1-independent survival mechanism for CASPASE-8 in αβ T cells

    doi: 10.1101/2025.03.27.645705

    Figure Lengend Snippet: Lymph nodes and spleen from the cohorts of mice described in were enumerated and analysed by flow. (A) Density plots are of CD4 vs CD8 by TCRβ + lymph node cells, CD62L vs CD44 by either CD4 + TCRβ + CD25 − or CD8 + TCRβ + subsets. Gates used to identify CD4 + naive, CM and EM subsets are labelled in red. Naive, CM and VM CD8 subsets were identified amongst CD8 + CD62L hi TCRβ + cells using CD49d and CD44 as indicated. (B) Bar charts summarised the total numbers of the indicated CD4 and CD8 subsets from total lymph nodes and spleen combined. (C-D) Density plots are of SSC vs Ki67 by the indicated subsets of CD4 and CD8 T cells in thymus and lymph nodes. Bar charts summarise the frequency of Ki67 + cells amongst the indicated subsets.

    Article Snippet: Panels used the following mAb: PE-Cy7-conjugated antibody against CD25 (eBioscience), PE-conjugated antibody against CD127 (ThermoFisher Scientific), BV785-conjugated CD44 antibody (Biolegend), BV421-conjugated antibody against CD4 (Biolegend), BUV395-conjugated antibody against CD8 (BD Biosciences), BV711-conjugated antibody against CD24 (BD Biosciences), PerCP-Cy5.5-conjugated antibody against TCR (Cambridge Biosciences), FITC-conjugated antibody against CD5 (eBiosciences), BV650-conjugated antibody against CD45.1 (Biolegend), FITC-conjugated antibody against CD45.2 (ThermoFisher Scientific), APC-conjugated antibody against CD49d (Biolegend), FITC-conjugated antibody against Ki67 (ThermoFisher Scientific).

    Techniques: